Decreased the cultured time by cellular stimulation with appropriate conditions of mechanical loading and light irradiation for applied to construct tissue-engineered bone made from patient’s stem cells
Bone is the hard tissue of the endoskeleton of the human body. As the age increase, the bone is prone to fracture under medical problems such as osteoporosis. Although the metal implant materials have been widely used but the bone can be damaged in connecting area between bone and implant materials occurred as bone has lower modulus of elasticity than implant material. Recently, Principle of tissue engineering is applied to construct tissue-engineered bone made from stem cells from a patient. However, cell culture process to make a large amount of calcium content to strengthen bone-tissue structure still takes too long time. Therefore, the aim of research is to decrease the cultured time by cellular stimulation with appropriate conditions of mechanical loading and light irradiation. The results of this study can evaluate effects of stimulation from experiments in both of cellular level (e.g., mRNA expression and protein production) and tissue level (e.g., Calcium content and mechanical properties). Finally, this study could be applied to construct tissue-engineered bone made from patient’s stem cells using a short time and would be used for orthopedic treatments as well.
Asst.Prof.Dr. Anak Khantachawana