Dujduan Waraho-Zhmayev, Ph.D.
Assistant Professor
+66 (0)2-470-9394
+66 (0)2-470-9391
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Research Interests:
  • Synthetic Biology
  • Protein Engineering (especially Antigen – Antibody Engineering)
  • Metabolic Engineering
  • In-vitro biosensors/immunosensors


  • 2009 Ph.D., Chemical Engineering, Cornell University, Ithaca, NY, USA
  • 2002 B. Eng., Chemical Engineering (1st class honor), RMIT University, Melbourne, Australia

Honors and Awards

  • 2010 – 2012 National Science Foundation Postdoctoral Research Fellowship (NSF Directorate for Education and Human Resources, USA)
  • 2004 – 2008 Graduate Research Fellowship (Cornell University, USA)
  • 1998 – 2008 Royal Thai Government Scholarship


Journal Articles
  • Akkapinyo, C., Khownarumit, P., Waraho-Zhmayev, D., Rungtiva P. Poo-arporn. 2020, Development of a multiplex immunochromatographic strip test and ultrasensitive electrochemical immunosensor for hepatitis B virus screening. Analytica Chimica Acta, 1095, 162-171.
  • Kamthong, A., Boonyalekha, P., Leelawattanachai, J., Waraho-Zhmayev, D. 2020. Application of the Synthetic Escherichia coli’s Twin-Arginine Translocation Pathway for the Detection of Intracellular Antibodies against Hepatitis C Viral Protease. International Journal of Bioscience, Biochemistry and Bioinformatics 10(1), 49-57.
  • Meksiriporn, B., Ludwicki, M.B., Stephens, E.A., Jiang, A., Lee, H.C., Waraho-Zhmayev, D. Kummer, L., Brandl F., Plückthun, A. and DeLisa, M.P. 2019. A survival-selection strategy for engineering synthetic binding proteins that specifically recognize post-translationally phosphorylated proteins. Nature Communications, 10(1):1830.
  • Mahajan, S.P., Meksiriporn, B., Waraho-Zhmayev, D. Weyant, K.B., Kocer, I., Butler, D.C., Messer, A., Escobedo, F.A. and DeLisa, M.P. 2018. Computational affinity maturation of camelid single-domain intrabodies against the nonamyloid component of alpha-synuclein. Scientific Reports, 8(1):17611.
  • Charoenpun, P., Leelawattanachai, J., Meechai, A., Waraho-Zhmayev, D. 2018. Production of bioactive trastuzumab and chimeric anti-VEGF antibody in the cytoplasm of Escherichia coli. IOP Conf. Ser.: Earth Environ. Sci., 185:012003.
  • Pitukdechatanakit, T., Khownarumit, P., Waraho-Zhmayev,D. and Poo-arporn,R.P. 2017. The effect of bovine serum albumin to platform of electrochemical immunosensor for hepatocellular carcinoma detection. International Journal of Advances in Science Engineering and Technology, 5(4), 63 – 67.
  • Poo-arporn, R.P., Pakapongpan, S., Khownarumit, P., Waraho-Zhmayev, D., Poo-arporn, Y. and Surareungchai, W. 2017. Development of mevalonic acid biosensor using amperometric technique based on nanocomposite of nicotinamide adenine dinucleotide and carbon nanotubes. Journal of the Electrochemical Society, 164 (7), B349-B355.
  • Thaiprasit, J., Kaewkamnerdpong, B., Waraho-Zhmayev, D., Cheevadhanarak, S. and Meechai, A. 2016. iDoRNA: An interacting domain-based tool for designing RNA-RNA interaction systems. Entropy, 18(3):83.
  • Waraho-Zhmayev, D., Meksiriporn, B., Portnoff, A.D., and DeLisa, M.P. 2014. Optimizing recombinant antibodies for intracellular function using hitchhiker-mediated survival selection. Protein Engineering Design and Selection, 27(10), 351 – 8.
  • Waraho-Zhmayev, D., Gkogka, L., Yu, T. and DeLisa, M.P. 2013. A microbial sensor for discovering structural probes of protein misfolding and aggregation. Prion, 7(2), 151 – 156.
  • Rocco, M.A., Waraho-Zhmayev, D. and DeLisa, M.P. 2012. Twin-arginine translocase mutations that suppress folding quality control and permit export of misfolded substrate proteins. Proc Natl Acad Sci USA, 109(33), 13392 – 7.
  • Waraho, D. and DeLisa, M.P. 2009. A highly efficient selection for intracellular protein protein interactions mediated by a unique bacterial hitchhiker transport mechanism.  Proc Natl Acad Sci USA, 106(10), 3692 – 7.
Book Chapters
  • Boock, J.T., Waraho-Zhmayev, D., Mizrachi, D., and DeLisa, M.P. 2015. Beyond the Cytoplasm of Escherichia coli: Localizing Recombinant Proteins Where You Want Them. Methods Mol Biol 1258: 79 – 97.
  • Waraho D and DeLisa MP. 2012. Identifying and optimizing intracellular protein-protein interactions using bacterial genetic selection. Methods Mol Biol 813: 125-43.
Conference Proceedings
  • Techasuwanphorn, P., Jullawateelert, J., Jangbua, P., Wattanachaisaereekul, S., Plongthongkum, P. Waraho-Zhmayev, D. 2019. Optimization of trastuzumab production in the cytoplasm of the engineered Esherichia coli     SHuffle strains.  The 2019 Biomedical Engineering International Conference (BMEiCON-2019), Ubon Ratchathani, Thailand, 19 – 22 November 2019. 1-6.
  • Chaiwanichaya, M., Chayaphum, A., Aungsutarn, A., Waraho-Zhmayev, D. and Meechai, A. 2017. Optimisation of Linalool Assay Conditions. The 7th International TIChE Conference (ITIChE 2017) "Innovative Chemical Engineering and Technology toward a Sustainable Future", Bangkok, Thailand, October 18-20, 2017, 92-94.
  • Khownarumit, P., Akkapinyo, C., Waraho-Zhmayev, D. and Poo-arporn, R.P. 2017. Electrochemical immunosensor for hepatitis B detection. The 2017 World Congress on Advances in Structural Engineering and Mechanics (ASEM17), Ilsan (Seoul), Korea, August 28-Sept 1, 2017. 1-9.
  • Akkapinyo, C., Waraho-Zhmayev, D., Khownarumit, P. and Poo-arporn, R.P. 2017. Development of multiplex hepatitis B test strip for hepatitis B screening. The 2017 World Congress on Advances in Structural Engineering and Mechanics (ASEM17), Ilsan (Seoul), Korea, August 28-Sept 1, 2017. 1-8.
  • Boonyalekha, P., Meechai, A., Tayapiwatana, C., Kitidee K. and Waraho-Zhmayev, D. 2017. Design and construction of a synthetic E. coli protease inhibitor detecting biomachine. The 39th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC’17), Jeju Island, Korea, July 11-15, 2017. 3580-3583.
  • Thanthongtip, S., Khownarumit, P., Waraho, D. and Palangsuntikul, R. 2015. Direct electrochemical detection of hemoglobin using screen-printed electrode modified with methylene Blue/graphene nanocomposite. Proceedings of Pure and Applied Chemistry International Conference (PACCON 2015), Bangkok, Thailand, January 21-23, 2015. 791-794.
  • Thaiprasit, J., Kaewkamnerdpong, B., Waraho, D., Cheevadhanarak, S. and Meechai, A. 2014. Domain-based design and platform of interacting RNAs: A promising tool in synthetic biology. Biomedical Engineering International Conference (BMEiCon) 7th, Fukuoka, Japan, November 26-28, 2014. 1 – 5.
  • Thaiprasit, J., Cheevadhanarak, S., Waraho, D. and Meechai, A. 2012. Conceptual design of RNA-RNA interaction based devices. Procedia Computer Science, 11, 139 – 148.
  • Thanasomboon, R., Waraho, D., Cheevadhanarak, S. and Meechai, A. 2012. Construction of synthetic Escherichia coli producing s-linalool. Procedia Computer Science, 11, 88 – 95.
  • Tawornsamretkit, I., Thanasomboon, R., Thaiprasit, J., Waraho, D., Cheevadhanarak, S. and Meechai, A. 2012. Analysis of metabolic network of synthetic Escherichia coli producing linalool using constraint-based modeling. Procedia Computer Science, 11, 24 – 35.
  • Hinthong, K., Kulthong, K., Waraho, D., Viravaidya, K., Kajitvichyanukul, P. and Maniratanachote, R. 2010. Comparative antibacterial effects of various types of ion-doped titanium dioxide under fluorescent light irradiation. IEEE NANO 2010, 10th International Conference on Nanotechnology, KINTEX, Seoul, Korea, August 17-20, 2010.
  • Sriratanakul, P., Kulthong, K., Phummirach, D., Waraho, D., Viriya-impikul, N., Viravaidya, K. and Maniratanachote, R. 2010. Cytotoxicity of titanate nanofilaments in human lung epithelial cell. NanoThailand 2010, Nanotechnology for a Sustainable World. Pathumthani, Thailand, November 18-20, 2010.
  • Thanasomboon, R., Waraho, D., Cheevadhanarak, S. and Meechai, A., 2010. Circuit design and synthesis Escherichia coli producing rose scent. The 22nd Annual Meeting of the Thai Society for Biotechnology, International Conference on Biotechnology for Healthy Living (TSB 2010), Thailand, October, 20-22.
  • Samruayphon, T., Jumlearnboonprakong, T., Somkieatprayool, P., Arintara, Y., Thanasomboon, R., Thaiprasit, J., Waraho, D. and Meechai, A., 2010. Design of synthetic plant as a xylene gas leak detector, The 22nd Annual Meeting of the Thai Society for Biotechnology, International Conference on Biotechnology for Healthy Living (TSB 2010), Thailand, October, 20-22.
  • Patent (International Publication Number WO 2007/024877) “Compositions and methods for analyzing protein interactions”
  • Patent (Thai Application Number 1701003852, applied on 7 July 2017) “Compositions & methods for isolating protease inhibitors based on the twin-arginine translocation pathway”


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